Study on the promotion of inflammation-induced premature brain injury repair by lactobacillus rhamnosus GG and related mechanisms

Title : Study on the promotion of inflammation-induced premature brain injury repair by lactobacillus rhamnosus GG and related mechanisms

Abstract:

Objective: This study aims to explore the role and mechanism of Lactobacillus rhamnosus GG (LGG) in alleviating brain damage caused by inflammation in premature mice.
Methods: Premature mice were induced by intraperitoneal injection of lipopolysaccharide (LPS). The groups included: LPS group: induced premature infants by LPS; antibiotic group: induced premature infants by LPS + antibiotic treatment (E18-P5); LGG group: induced premature infants by LPS + antibiotic treatment (E18-P5) + LGG. observe the composition of intestinal microbiota, pathological changes and immunostaining of brain tissue in the three groups of mice when P25.
Results: LGG regulated the composition of intestinal microbiota, reduced the proportion of harmful bacteria, and increased the levels of bile salts and short-chain fatty acids in fecal samples. Compared with the LPS group and the antibiotic group, the brain tissue structure of the LGG group was more complete, the number of brain cells was greater, and the morphology was more mature. LGG treatment also reduced the activation of cortical microglia and decreased the levels of pro-inflammatory cytokines in brain tissue. During this process, LGG regulated the expression of the FGF23 gene, which is closely related to neurodevelopment and injury.
Conclusion: LGG intervention may alleviate neuroinflammation through the microbiota-gutbrain axis, reduce neuronal apoptosis, and improve brain damage caused by inflammation in premature infants. FGF23 may be a key regulatory gene in this process. These findings suggest that inflammation-induced brain damage in premature infants and neuroinflammatory responses are influenced by the intestinal microbiota, providing opportunities for therapeutic intervention through targeting the early colonization and development of the gut microbiota. This inspires us that probiotics may serve as a promising adjunctive therapy for improving brain damage in premature infants.

Biography:

Professor Liu is a neonatologist, doctoral degree tutor, the founder and discipline leader of Department of Neonatology，the First Affiliated Hospital of Xi 'an Jiaotong University, visiting scientist at Medical school of Harvard University. She obtained her doctoral degree from Xi'an Jiaotong University in 2009. Her research focuses on the brain development and injury, especially in premature infants.She has previously led four projects funded by the National Natural Science Foundation of China and published more than 50 research articles in SCI(E) journals. And She was awarded the "China Neonatal Physician Award"、"National Science and Technology Award". She is Vice President of Neonatal Branch of Chinese Medical Doctor Association, deputy chairman of the special Committee on Child health and chairman of the Professional Committee on early Childhood Health Development, Head of the neonatal Cooperation Group in Northwest China and chairman of the Expert Committee, Chairman of neonatologists Branch of Shaanxi Provincial Medical Association. And she is also a member of the editorial boards of more than ten domestic and internationl magazines.